What is the Full form of HPLC? HPLC stands for High-performance Liquid Chromatography or High-Pressure Liquid Chromatography. What is the HPLC Principle? It is a chromatographic technic used to separate the components from a mixture of a solution by using a solid stationary phase and a liquid mobile phase. How many types of…
Analytical Instrument Qualification (AIQ) and system suitability testing (SST) are both important processes in the pharmaceutical industry that ensure the accuracy, reliability, and consistency of analytical instruments used for testing drug products. However, there are some key differences between these two processes. 1. Purpose: – AIQ: The primary purpose of AIQ is to establish…
Discovering straightforward tips for success in optimizing HPLC methods: 1. Use the appropriate column for your analytes and separation goals. 2. Optimize the mobile phase composition, including solvent type and concentration. 3. Adjust the pH of the mobile phase if necessary. 4. Optimize the flow rate to achieve optimal separation and resolution. 5. Use the…
1. Method Development This is the initial stage where the test method is developed based on the specific requirements of the pharmaceutical product. It involves determining the analytical technique, selecting appropriate instruments and reagents , and establishing the method parameters. 2. Method Validation Once the test method is developed, it needs to be validated…
pH and pKa are two important concepts in chemistry that are often confused. pH pKa The relationship between pH and pKa pH = pKa + log(A-/HA) Where, Is pKa the same as pH? It is important to remember that when the pH equals to pKa value, the proportions of conjugate base…
Non-sink conditions refer to situations where the concentration of the drug substance in the dissolution medium is not significantly lower than its solubility. While sink conditions are commonly preferred in dissolution testing to ensure rapid and complete drug dissolution, there are certain instances where non-sink conditions can be useful. Here are a few examples:…
Sink conditions refer to a state in which the dissolution medium has sufficient volume to ensure the drug dissolves rapidly without reaching saturation throughout the duration of the test. Sink conditions = V/Vsat ≥ 3 Where, Volume of Dissolution Medium The volume of the dissolution medium should be at least 3-10 times the saturation…
