Difference Between Disinfection and Sterilization

Disinfection

  • It kills only vegetative cells and not the spores.
  • In this, the number of harmful microbes is minimized to a negligible level.
  • Wounds are disinfected – with agents such as hydrogen peroxide or rubbing alcohol.
  • Disinfection only reduces the effect of microbes.
  • Chemical methods are used for disinfection .
  • Phenol, alcohol, chlorine, iodine are some of the disinfecting agents.
  • Only adequate cleanliness.
  • Used in daily life.
  • For eg., pasteurization, disinfecting urinals, etc.

Sterilization

  • It kills both vegetative cells and spores.
  • In this, the medium is made completely free from all microbes.
  • Wounds cannot be sterilized – as it may kill surrounding healthy cells.
  • Sterilization completely rids microbes from the surface
  • Combination of heat, irradiation, high pressure, chemical and physical methods are used for sterilization
  • High temperatures, steam, radiation, filtration are some of the sterilization techniques.
  • Extreme cleanliness
  • Used primarily for medical and research purposes.
  • For eg., sterilization of instruments used during surgery by autoclaving.

Disinfection and sterilization have a few things in common. These include:

  • Both are used to decontaminate objects and surfaces
  • Both processes can kill (most) harmful microbes.


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Resource Person: Ch/Ahmed Mahmoud

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