HPLC

Common Interview Questions and Answers on HPLC
| |

Common Interview Questions and Answers on HPLC

What is the Full form of HPLC? HPLC stands for High-performance Liquid Chromatography or High-Pressure Liquid Chromatography.   What is the HPLC Principle? It is a chromatographic technic used to separate the components from a mixture of a solution by using a solid stationary phase and a liquid mobile phase.   How many types of…

Tips for Optimizing HPLC Method in Pharmaceutical Analysis
|

Tips for Optimizing HPLC Method in Pharmaceutical Analysis

Discovering straightforward tips for success in optimizing HPLC methods: 1. Use the appropriate column for your analytes and separation goals. 2. Optimize the mobile phase composition, including solvent type and concentration. 3. Adjust the pH of the mobile phase if necessary. 4. Optimize the flow rate to achieve optimal separation and resolution. 5. Use the…