Calibration of Volumetric Flasks
Weigh a clean & dry volumetric flask. Calibrate the flask by filling it with recently boiled and cooled distilled water at room temperature up to the mark and weigh the flask. Determine the volume in ml by the formula;
Volume (in ml) = (Weight in g / 0.99602)
Where, 0.99602 g is the weight of 1 ml water determined at temperature 25°C.
For the volumetric flasks having capacity more than 100 ml, dispense the water from 100 ml burette (class A) in multiple steps if required and note the final volume (i.e. total volume dispensed).
Calibrate the one mark pipette / graduated pipette/burette :Fill it with recently boiled and cooled distilled water at room temperature up to the mark. Collect the water from the pipette into a clean, dry previously weighed beaker [For burette – drain the water up to the lower mark]. Take the weight of beaker with water.
Check the weight of water by subtracting the wt. of empty beaker from the weight of beaker with water.
Determine the volume in ml by the formula;
Volume (in ml) = (Weight in g / 0.99602)
Where 0.99602 g is the weight of 1 ml water determined at temperature 25°C.
Stage/ frequency: Initially when volumetric glassware is purchased.
When the volumetric glassware does not comply with the requirement as specified above, the volumetric glassware should be returned back.
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Resource Person: Susmita
