Calculation

Calculation of Peak Purity in HPLC
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Calculation of Peak Purity in HPLC

The calculation of peak purity in HPLC (High-Performance Liquid Chromatography) is typically performed using a photodiode array (PDA) detector, which can detect absorbance at multiple wavelengths simultaneously. Steps : Collect Spectral Data Across the Peak: During HPLC analysis, a PDA detector collects the UV-visible spectrum at different points across the peak (start, apex, and end…

Air Changes Calculation Formula for an Injectable Area

Air Changes Calculation Formula for an Injectable Area

The air changes calculation formula for an injection department, or any cleanroom environment, is as follows: Air Changes Calculation Formula ACH = (Total Airflow) / (Room Volume) Where: Example Calculation: Suppose we have an injection department with the following parameters: Using the formula: ACH = (10,000 m³/h) / (300 m³) = 33.33 air changes per…