HPLC

The calculation of peak purity in HPLC (High-Performance Liquid Chromatography) is typically performed using a photodiode array (PDA) detector, which can detect absorbance at multiple wavelengths simultaneously. Steps : Collect Spectral Data Across the Peak: During HPLC analysis, a PDA detector collects the UV-visible spectrum at different points across the peak (start, apex, and end…