Why Do We Incubate BET Reaction Tubes at 37°C for 60 Minutes?
In the Bacterial Endotoxins Test (BET), incubating reaction tubes at 37°C ± 1 for exactly 60 ± 2 minutes is a key step. But why such specific conditions? Let’s dive in. 1. Optimal Enzyme Activity The heart of BET lies in the Limulus Amebocyte Lysate (LAL) reagent, sourced from horseshoe crab blood. This enzyme reacts…
